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The Monsters, Inc. Laugh Floor is an interactive show that uses "digital puppetry". Characters shown on a screen are played controlled in real time by back-stage puppeteers. The attraction is based on the Disney-Pixar movie Monsters, Inc. The Laugh Floor takes place in the fictional world of Monstropolis.

The first act at the Monsters Inc. Laugh Floor is a two headed yellow Monster named Mac and Jeeves, though the monster can go by other names depending on the cast member performing. Mac (the smaller of the two heads) boasts that he is a master of human world geography and that he knows every capital in the world. Jeeves is skeptical, so to prove his knowledge he asks the humans selects a human audience member to name name any country. After they do, Mac's answer is usually a humorous side-step of the question (ie. , such as saying the capital of Canada is "C". Following this bit, a large map descends from behind Mac and Jeeves, and they ask the humans audience to raise their hands if they "you know where they (the humans) are fromyou live. " After a guest says names their hometown, Mac will give a completely wrong (albeit humorous) description of the location.

Mac: "Venezuela is a tiny Arctic country located in the middle of the North Arctic Ocean, famous for ski resorts and the Eiffel Tower!"

After repeating this routine with a couple of guests, Mac and Jeeves then decide to recreate the story of Monsters Inc. using guests from the Audienceaudience. Different audience members are selected to portray characters from the movie and they are given silly facial expressions to make. After all of the characters have been selected, Mac and Jeeves retell the story very quickly, and the guests have to make the expressions fast in order to keep up. Finally, Roz pushes the trap door button again, and it's time for the second act.

Following Mac and Jeeves a purple monster named Buddy Boil takes the stage. He claims he's a mind reader and then asks a random audience member to think of a number. He asks the volunteer to say the number out loud, closing his eyes - not his ears - so that he will not hear it. Buddy then tells it the number to the rest of the group. Everyone knows he can hear the number so when Buddy guesses it right and looks surprised, the audience usually laughs. At this point Roz clicks the button, which triggers a trap door below Buddy. Sometimes after falling Buddy humorously asks the same person to pick another number and he repeats the same shtick.

The final act of the Laugh Floor is none other than Mike's nephew Marty. Marty looks like a smaller orange version of Mike, and his act is pretty much a stand up routine. Marty starts out with a monologue. His first couple jokes usually involve his mother ("She really does have eyes on the back of her head") or some kind of pun ("I have a test tomorrow but iI'm not" worried...It's an eye exam!"). After he has finished his monologue, Marty reads some of the jokes that guests sent in. He reads three or four of those before going back to his own jokes. The final jokes of his routine usually require some participation. Marty usually calls on young children to help him with these final jokes.